Eps 428: dek
— The too lazy to register an account podcast
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Anne Williams
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However, there is no evidence that IHC can be detected in normal tissue in the presence of the DEK protein. 66 cases showed a positive staining of DEk protein, while 32 cases or 39% showed that the DE-K protein was significantly higher than normal tissue .
IRs, the researchers found that IR is conserved in dormant adult stem cells, suggesting that DEK plays an important role in the resting and exit of stem cells. Dek protein is not present in QSCs and the results were published in the Journal of the American Medical Association
The presence of DEK in QSCs and its presence in dormant adult stem cells implies an important role in the resting and excretion of these cells from the body and into the bloodstream.
Recent findings have shown that DEK as an oncoprotein regulates the repair and repair of cells in epithelial cells as well as oncogenes such as E6, E7 and E8. The expression of DEk promotes proliferation and transformation of epithelial cells, inhibits normal cell differentiation, suppresses the p53 pathway that induces apoptosis, and acts as an anti-aging agent by inhibiting the oncogene E 6-E7. On the other hand, DE K depletion can trigger apoptotic senescence of the cells and sensitize the cells to chemotherapy and genotoxic substances.
To further investigate the function of DEK in lung cancer cells, a plasmid-coding CDS - labeled with DEk - was used to regulate and regulate the expression levels of DeK within the lung cancer cell line a549 and these in turn to further investigate its role in the development of cancer cells.
Expression levels of DEK mRNA and protein were analyzed using QRT-PCR and expression levels of DE K mRNA protein were analyzed 48 post-transfection. The results showed that the expression of DeK was regulated and that transactions with the plasmid coding sign DEk caused its expression within the a549 cells.
After transfection with Mir489, dek protein expression in a549 cells was reduced. Bioinformatics analyses showed that the expression of DEK mRNA and protein increases with the presence of the plasmid and its interaction with dek protein.
SPOP mutants predominantly negatively affected the ubiquitylation of a subset of proteins. DEK trim24-603406 developed into an effector substrate that was consistently upregulated by SPOP mutants.
The DEK stabilization promoted the invasion of the prostate epithelial cells, which DEK has implied as an oncogenic effector .
Since DEK is a poly-ADP-ribose acceptor for apoptosis, we investigated whether its downward regulation would also affect DNA strand breaks caused by exposure to the ABT-8888 replication inhibitor.
In the case of CPT, DNA is known to form as a DNA strand break, but this interaction is only detectable when the fork is still processed. Overall, our data show that the speed of the challenged replication fork in the presence of ABT-8888 depends on DEK expression levels, and suggest that a regulatory network that includes DEk and PARP1 / 2 modulates the fork speed.
We investigated the possibility that DEK is directly related to the replication fork, in particular to ABT-8888 and its interaction with PARP1 / 2.Let in the presence of CPT and ABt-8888 and the interaction between DEk and PARp1 / 2.
PCNs were visualized by indirect immunofluorescence and U2 OS GFP - treated DEK cells were treated. Based on this data, we cannot rule out that DEk is part of the replicator, which makes it possible that the direct interaction between ABt-8888 and PARp1 / 2, which promotes the progression of the replication fork, is through direct interaction. At present, the presence or absence of a direct relationship between CPT and DE K in the same cell cannot be excluded by varying the number of cells in a cell, taking into account the possible role of DE k in its interaction with PARP1 / 2.